Fine Needle Aspirates of Enlarged Lymph Nodes. Role of Ziehl Neelsen Stain in the Diagnosis of Tuberculosis
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چکیده
Background: Lymph node tuberculosis (LNTB) is the leading form of extra pulmonary tuberculosis and common in our country. Acid Fast Bacilli (AFB) detection by Ziehl-Neelsen (ZN) method in the fine needle aspirate from these lymph nodes is confirmatory. Objective: To assess the diagnostic utility of Ziehl-Neelsen staining in lymphadenopathy cases, since it is commonly used method in the diagnosis of TB. Methods: A total of 150 cases of tuberculous lymphadenitis diagnosed by fine needle aspiration cytology (FNAC) were included in this study. Smears of the aspirates from all these cases were stained with Ziehl-Neelsen stain and examined by the light microscope. Results of Acid Fast Bacilli by Ziehl-Neelsen and cytomorphology by fine needle aspiration cytology were compared. Results: The classic cytomorphological pattern of tuberculous lymphadenitis (epithelioid granulomas, Langhan’s giant cells and caseous necrosis) by Fine Needle Aspiration Cytology was seen in 150 cases. These were divided into three categories among which maximum cases (80.95%) were confirmed in the smears showing caseous material only. By concluding the results routine Ziehl-Neelsen staining confirmed Acid Fast Bacilli in 90 (60 %) cases. Conclusion: Ziehl-Neelsen staining if coupled with fine needle aspiration cytology of suspected tuberculous lymphadenopathy cases can confirm diagnosis of tuberculosis.
منابع مشابه
Correlation of Various Techniques in Diagnosis of Tuberculous Lymphadenitis on Fine Needle Aspiration Cytology
Objective. To study the correlation of cytomorphological features in fine needle aspiration smears from patients suspected of having tuberculous lymphadenitis with Ziehl-Neelsen staining (ZN), auramine-rhodamine staining (ARS), and autofluorescence (AF). Methods. A total of 145 lymph nodes were aspirated, 3 air-dried smears were stained with Giemsa, Ziehl-Neelsen, and auramine-rhodamine stains,...
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